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6-Fluoropyridoxol: a novel probe of cellular pH using 19F NMR spectroscopy
Vimal D. Mehta, Padmakar V. Kulkarni, Ralph P. Mason,
Anca Constantinescu, Sivasubramanian Aravind, Naresh Goomer
and Peter P. Antich.
Department of Radiology,
UT-Southwestern Medical Center,
Dallas, TX
6-Fluoropyridoxol was evaluated as an intra-cellular pH indicator. This molecule exhibits exceptional sensitivity to changes in pH (~ 10 ppm acid/base shift) and a pKa ~ 8.2 appropriate for physiological investigations. Using 19F NMR spectroscopy we determined both intra- and extra-cellular pH in whole blood and confirmed the measurements using traditional techniques: ion-electrodes and 31P NMR spectroscopy.
For Further Information Contact: RALPH
P. MASON, Ph.D.
Page Maintained by: Lan Jiang,
M.Sc.
Page Created by: Robert Bollinger II
Copyright ©2001 UT Southwestern
Last Updated:
Thursday, September 20, 2001
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